Product name HEPES buffer (1×with Calcium-Magnesium) HEPES buffer (1×, containing calcium and magnesium)

Product specifications 500mL

Reagent storage Transport at room temperature, store at 2-8℃

Validity period: 12 months (use as soon as possible after opening the lid)

Product Introduction 

HEPES is 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, which is non-toxic to cells. HEPES is a non-ionic amphoteric buffer, which can effectively control the pH in the range of 6.8 to 8.2, especially at pH 7.2 to 7.4. It has good buffering capacity. The final concentration is generally 10 to 50 mmol/L. mmol/L HEPES can achieve better buffer capacity.

Phygene HEPES buffer (1×, containing calcium and magnesium) is a commonly used solution in cell-cell adhesion experiments. It is mainly composed of HEPES, sodium chloride, potassium chloride, phosphate, glucose, etc., and contains Ca2+, Mg2+, pH7.4, sterilized by filtration. It is often used to clean tissues or cells (especially embryonic cells) during long-term aggregation culture to protect the cadherin of cells without affecting the formation of cell aggregation.

This product is sterilized by 0.2μm filter

Use reference 

1. Mainly used for washing cells, similar to PBS for direct use.

2. If it is a cell, just wash it directly. If it is a tissue, the tissue should be cut out, placed in pre-cooled HEPES buffer (1 ×, containing calcium and magnesium), and cut into small pieces, collected with the minimum amount of buffer, and transferred to a BSA-coated petri dish In, use scissors to cut the tissues for downstream cell culture experiments.

Precautions 

1. Pay attention to aseptic operation and try to avoid contamination.

2. For your safety and health, please wear lab coats and disposable gloves.

人工模拟生物体液试剂和仪器的先驱

Biochemazone™是一家不断发展的生物科技公司，提供高品质、纯化的人工生物化学品、试剂和解决方案，如模拟唾液、模拟尿液、模拟汗液等，并开发学术研究单位、大学、医院、工业、政府和私人实验室。

我们的生化试剂经过全面测试，可满足对卓越品质、不妥协生物的研发需求，以具有竞争力的价格准确模拟真正的生物解决方案活动。

Biochemazone™在科学界建立了良好的声誉，其声誉建立在创新产品、客户服务和对细节的关注上。我们的质量声誉是在市场上取得有利地位以及与制造顶级合成生物产品（如人造唾液、人造尿液、人造皮脂、人造汗液、人造汗液、人造血清、人工血液、人工精液、人工耳垢等等……

欢迎消费品测试实验室和制造商联系 Biochemazone™或本地代理商以了解人工模拟生物流体液体和生化试剂的需求。